However, there is also evidence showing that ty- 5 is a loss-of-function mutation that likely occurred in cultivated tomato (Levin et al. The ty- 5 gene is likely derived from a complex of S. The recessively inherited ty- 5 gene, first identified in the breeding line TY172 and later found in material derived from “Tyking,” was mapped to chromosome 4 (Anbinder et al. chilense, was mapped to chromosome 3 (Ji et al. ( 2013) demonstrated that Ty- 1 and Ty- 3 are alleles of the same gene. chilense and mapped to nearby positions on chromosome 6 (Ji et al.

Ty- 1 and Ty- 3 were each derived from S. So far, as many as five resistance loci have been mapped, i.e., the dominant genes including Ty- 1, Ty- 2, Ty- 3, Ty- 4, and recessive gene ty- 5 (Zamir et al. Species that have demonstrated resistance include S. Cultivated tomato is susceptible to TYLCV, so breeding efforts rely on the transfer of resistance genes from wild tomato relatives. Thus, deployment of resistant cultivars offers an attractive method to control these diseases. Also, whitefly resistance to the used chemicals has been reported (Antignus et al. Whitefly control measures such as the use of insecticides and/or fine-mesh screens or UV-absorbing plastic films/screens can limit disease damage, but epidemics can still occur. These viruses cause serious losses to tomato ( Solanum lycopersicum L.) production in many tropical and subtropical regions in the world (Ji et al. Tomato-infecting begomoviruses, including the monopartite tomato yellow leaf curl virus (TYLCV) and numerous bipartite viruses, are transmitted by the adult sweet potato whitefly, which is also known as the silverleaf whitefly ( B. Several markers very tightly linked to the Ty- 2 locus are presented and useful for marker-assisted selection in breeding programs to introgress Ty- 2 for begomovirus resistance.

Results indicate that the NBS gene family present in the Ty- 2 region is likely not responsible for the Ty- 2-conferred resistance and that two candidate genes might play a role in the Ty- 2-conferred resistance. Candidate genes predicted based on tomato genome annotation were analyzed by RT-PCR and virus-induced gene silencing. Due to the small interval, fluorescence in situ hybridization analysis failed to clarify whether recombination suppression is caused by chromosomal rearrangements. No recombinants were identified between TG36 and C2_At3g52090, a region of at least 115 kb, indicating severe recombination suppression in this region. Molecular marker analysis of recombinants and TYLCV evaluation of progeny from these recombinants localized Ty- 2 to an approximately 300,000-bp interval between markers UP8 (51.344 Mb) and M1 (51.645 Mb). In the present study, approximately 11,000 plants were screened and nearly 157 recombination events were identified between the flanking markers C2_At1g07960 (82.5 cM, physical distance 51.387 Mb) and T0302 (89 cM, 51.878 Mb). glabratum accession “B6013 ,” that confers resistance to TYLCV was previously mapped to a 19-cM region on the long arm of chromosome 11. Resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (TYLCV), have been introgressed to cultivated tomato ( Solanum lycopersicum) from wild tomato accessions.

Tomato 2 leaves wageningen